Protein hydrolysates from poultry offer health benefits like antidiabetic and antioxidant properties. This study focused on collagen-rich hydrolysates from mechanically deboned chicken residues (MDCR), a by-product of the poultry industry. Using enzymatic protein hydrolysis (EPH), MDCR was divided into water phase, and solid phase (containing collagen-rich fraction). In the first EPH step, the MDCR was treated with either Endocut 07L and Exocut TRL to enriched the collagen in the substrate. In second two other proteases were selected to produce collagen peptides on those sediements Flavorpro 766 MDP and Bromelain respectively. The collagen peptides were fractionated based on the hydrodynamic volumne (HPLC-SEC), and the antidiabetic acitivity between the fractions have been measured to determine the most active fraction.